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Increasing acetate intake regulated gut-brain serotonin metabolism. a Mice experimental design. b Body weight changes before behavioral experiments (n = 10 per group). c The effect size of percent PPI impairments at 75, 80 and 85 dB. The percent scores of distances ( d ) and time ( e ) spent in target quadrant of MWM test. f-q Western blot results of proteins expression in colon ( f ) and PFC ( g ). The quantification of Tph1 ( h ), Ddc ( i ), Maoa ( j ), Sert ( k ), Ffar3 ( l ) and Ffar2 ( m ) in the colon as well as <t>Tph2</t> ( n ), Ddc ( o ), Maoa ( p ) and Sert ( q ) in the PFC were performed. Data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns depicts no significant difference.
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Increasing acetate intake regulated gut-brain serotonin metabolism. a Mice experimental design. b Body weight changes before behavioral experiments (n = 10 per group). c The effect size of percent PPI impairments at 75, 80 and 85 dB. The percent scores of distances ( d ) and time ( e ) spent in target quadrant of MWM test. f-q Western blot results of proteins expression in colon ( f ) and PFC ( g ). The quantification of Tph1 ( h ), Ddc ( i ), Maoa ( j ), Sert ( k ), Ffar3 ( l ) and Ffar2 ( m ) in the colon as well as <t>Tph2</t> ( n ), Ddc ( o ), Maoa ( p ) and Sert ( q ) in the PFC were performed. Data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns depicts no significant difference.
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Increasing acetate intake regulated gut-brain serotonin metabolism. a Mice experimental design. b Body weight changes before behavioral experiments (n = 10 per group). c The effect size of percent PPI impairments at 75, 80 and 85 dB. The percent scores of distances ( d ) and time ( e ) spent in target quadrant of MWM test. f-q Western blot results of proteins expression in colon ( f ) and PFC ( g ). The quantification of Tph1 ( h ), Ddc ( i ), Maoa ( j ), Sert ( k ), Ffar3 ( l ) and Ffar2 ( m ) in the colon as well as <t>Tph2</t> ( n ), Ddc ( o ), Maoa ( p ) and Sert ( q ) in the PFC were performed. Data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns depicts no significant difference.
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Increasing acetate intake regulated gut-brain serotonin metabolism. a Mice experimental design. b Body weight changes before behavioral experiments (n = 10 per group). c The effect size of percent PPI impairments at 75, 80 and 85 dB. The percent scores of distances ( d ) and time ( e ) spent in target quadrant of MWM test. f-q Western blot results of proteins expression in colon ( f ) and PFC ( g ). The quantification of Tph1 ( h ), Ddc ( i ), Maoa ( j ), Sert ( k ), Ffar3 ( l ) and Ffar2 ( m ) in the colon as well as <t>Tph2</t> ( n ), Ddc ( o ), Maoa ( p ) and Sert ( q ) in the PFC were performed. Data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns depicts no significant difference.
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https://www.bioz.com/result/rabbit anti- tph2 antibody/product/Novus Biologicals
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Increasing acetate intake regulated gut-brain serotonin metabolism. a Mice experimental design. b Body weight changes before behavioral experiments (n = 10 per group). c The effect size of percent PPI impairments at 75, 80 and 85 dB. The percent scores of distances ( d ) and time ( e ) spent in target quadrant of MWM test. f-q Western blot results of proteins expression in colon ( f ) and PFC ( g ). The quantification of Tph1 ( h ), Ddc ( i ), Maoa ( j ), Sert ( k ), Ffar3 ( l ) and Ffar2 ( m ) in the colon as well as Tph2 ( n ), Ddc ( o ), Maoa ( p ) and Sert ( q ) in the PFC were performed. Data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns depicts no significant difference.

Journal: medRxiv

Article Title: Acetate-producing Prevotella copri aggravates schizophrenia behaviors by modulating serotonin metabolism

doi: 10.1101/2025.03.25.25324621

Figure Lengend Snippet: Increasing acetate intake regulated gut-brain serotonin metabolism. a Mice experimental design. b Body weight changes before behavioral experiments (n = 10 per group). c The effect size of percent PPI impairments at 75, 80 and 85 dB. The percent scores of distances ( d ) and time ( e ) spent in target quadrant of MWM test. f-q Western blot results of proteins expression in colon ( f ) and PFC ( g ). The quantification of Tph1 ( h ), Ddc ( i ), Maoa ( j ), Sert ( k ), Ffar3 ( l ) and Ffar2 ( m ) in the colon as well as Tph2 ( n ), Ddc ( o ), Maoa ( p ) and Sert ( q ) in the PFC were performed. Data are represented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns depicts no significant difference.

Article Snippet: The information for antibodies is as follows: Tph1 (D10C10) Rabbit mAb (#12339, Cell Signaling Technology), Tph2 (D3E5I) XP® Rabbit mAb (#51124, Cell Signaling Technology), Ddc (D6N8N) Rabbit mAb (#13561, Cell Signaling Technology), Maoa (E3L3B) Rabbit mAb (#75330, Cell Signaling Technology), anti-serotonin transporter antibody (EPR26259-61, abcam), FFAR3 antibody (CSB-PA008939, Cusabio Technology), FFAR2 Polyclonal antibody (19952-1-AP, Proteintech Group), β-Actin (13E5) Rabbit mAb (#4970, Cell Signaling Technology).

Techniques: Western Blot, Expressing